Comparison of 6 automated assays for total and free prostate-specific antigen with special reference to their reactivity toward the WHO 96/670 reference preparation
Identifieur interne : 001702 ( Main/Exploration ); précédent : 001701; suivant : 001703Comparison of 6 automated assays for total and free prostate-specific antigen with special reference to their reactivity toward the WHO 96/670 reference preparation
Auteurs : Sheila A. R. Kort [Pays-Bas] ; Frans Martens [Pays-Bas] ; Hilde Vanpoucke [Belgique] ; Hans L. Van Duijnhoven [Pays-Bas] ; Marinus A. Blankenstein [Pays-Bas]Source :
- Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Biochimie.
English descriptors
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Abstract
Background: Prostate-specific antigen (PSA) assays have historically produced different results. Our aim was to investigate the comparability of assay results of selected commercially available assay methods designed to measure total, free, or complexed PSA (tPSA, fPSA, and cPSA). Methods: We measured tPSA, fPSA, and cPSA in 70 samples and in the WHO PSA 96/670 reference preparation with 6 assays (Beckman-Coulter Access, Abbott ARCHITECT and AxSYM, Bayer Advia Centaur, DPC IMMULITE 2000, and Roche Modular Analytics E170). We also calculated the fPSA/tPSA ratio. Results: The mean deviations from the expected tPSA and fPSA values for the WHO 96/670 reference preparation were 0.37 (range, 0.01-1.32) and 0.19 (range, 0.05-0.49) μg/L, respectively. When plotted against the expected WHO 96/670 reference preparation value, regression slopes varied from 0.99 to 1.22 and r2 from 0.9996 to 1.000. When total PSA was measured in mixtures of sera with high and low tPSA concentrations, the mean (SD) slope of regression of different assays against an in-house method was 1.04 (0.09). In these specimens, the fPSA/tPSA ratio was 0.11-0.14 with different methods. The tPSA and fPSA values in patient samples measured in different assays and plotted against ARCHITECT gave regression slopes from 0.88 to 0.97. The results of the studied assays for tPSA in serum samples agreed within 15%, from each other, and all results for the WHO 96/670 reference preparation were within 6.8% (confidence interval, 1.7%-15.2%) of the expected value. The results for fPSA were more diverse. Conclusions: Differences among PSA assays appear to have decreased since introduction of the WHO 96/670 reference preparation, but further efforts are needed to harmonize fPSA assays.
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R." last="Kort">Sheila A. R. Kort</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Endocrine Laboratory, Department of Clinical Chemistry, VU University Medical Center</s1><s2>Amsterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Martens, Frans" sort="Martens, Frans" uniqKey="Martens F" first="Frans" last="Martens">Frans Martens</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Endocrine Laboratory, Department of Clinical Chemistry, VU University Medical Center</s1><s2>Amsterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Vanpoucke, Hilde" sort="Vanpoucke, Hilde" uniqKey="Vanpoucke H" first="Hilde" last="Vanpoucke">Hilde Vanpoucke</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratory of Clinical Chemistry, H. Hartziekenhuis</s1><s2>Roeselare-Menen vzw</s2><s3>BEL</s3><sZ>3 aut.</sZ></inist:fA14><country>Belgique</country><wicri:noRegion>Roeselare-Menen vzw</wicri:noRegion></affiliation></author><author><name sortKey="Van Duijnhoven, Hans L" sort="Van Duijnhoven, Hans L" uniqKey="Van Duijnhoven H" first="Hans L." last="Van Duijnhoven">Hans L. Van Duijnhoven</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Algemeen Klinisch Laboratorium, Elkerliek Ziekenhuis</s1><s2>Helmond</s2><s3>NLD</s3><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>Helmond</wicri:noRegion></affiliation></author><author><name sortKey="Blankenstein, Marinus A" sort="Blankenstein, Marinus A" uniqKey="Blankenstein M" first="Marinus A." last="Blankenstein">Marinus A. Blankenstein</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Endocrine Laboratory, Department of Clinical Chemistry, VU University Medical Center</s1><s2>Amsterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Automatic analysis</term><term>Biochemistry</term><term>Clinical biology</term><term>Comparative study</term><term>Free form</term><term>Molecular biology</term><term>Preparation</term><term>Prostate specific antigen</term><term>Reactivity</term><term>Reference</term><term>Tumoral marker</term><term>WHO</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Etude comparative</term><term>Analyse automatique</term><term>Forme libre</term><term>Antigène spécifique prostate</term><term>Marqueur tumoral</term><term>Référence</term><term>Réactivité</term><term>OMS</term><term>Préparation</term><term>Biochimie</term><term>Biologie clinique</term><term>Biologie moléculaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Prostate-specific antigen (PSA) assays have historically produced different results. Our aim was to investigate the comparability of assay results of selected commercially available assay methods designed to measure total, free, or complexed PSA (tPSA, fPSA, and cPSA). Methods: We measured tPSA, fPSA, and cPSA in 70 samples and in the WHO PSA 96/670 reference preparation with 6 assays (Beckman-Coulter Access, Abbott ARCHITECT and AxSYM, Bayer Advia Centaur, DPC IMMULITE 2000, and Roche Modular Analytics E170). We also calculated the fPSA/tPSA ratio. Results: The mean deviations from the expected tPSA and fPSA values for the WHO 96/670 reference preparation were 0.37 (range, 0.01-1.32) and 0.19 (range, 0.05-0.49) μg/L, respectively. When plotted against the expected WHO 96/670 reference preparation value, regression slopes varied from 0.99 to 1.22 and r2 from 0.9996 to 1.000. When total PSA was measured in mixtures of sera with high and low tPSA concentrations, the mean (SD) slope of regression of different assays against an in-house method was 1.04 (0.09). In these specimens, the fPSA/tPSA ratio was 0.11-0.14 with different methods. The tPSA and fPSA values in patient samples measured in different assays and plotted against ARCHITECT gave regression slopes from 0.88 to 0.97. The results of the studied assays for tPSA in serum samples agreed within 15%, from each other, and all results for the WHO 96/670 reference preparation were within 6.8% (confidence interval, 1.7%-15.2%) of the expected value. The results for fPSA were more diverse. Conclusions: Differences among PSA assays appear to have decreased since introduction of the WHO 96/670 reference preparation, but further efforts are needed to harmonize fPSA assays.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>Pays-Bas</li></country><region><li>Hollande-Septentrionale</li></region><settlement><li>Amsterdam</li></settlement></list><tree><country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Kort, Sheila A R" sort="Kort, Sheila A R" uniqKey="Kort S" first="Sheila A. R." last="Kort">Sheila A. R. Kort</name></region><name sortKey="Blankenstein, Marinus A" sort="Blankenstein, Marinus A" uniqKey="Blankenstein M" first="Marinus A." last="Blankenstein">Marinus A. Blankenstein</name><name sortKey="Martens, Frans" sort="Martens, Frans" uniqKey="Martens F" first="Frans" last="Martens">Frans Martens</name><name sortKey="Van Duijnhoven, Hans L" sort="Van Duijnhoven, Hans L" uniqKey="Van Duijnhoven H" first="Hans L." last="Van Duijnhoven">Hans L. Van Duijnhoven</name></country><country name="Belgique"><noRegion><name sortKey="Vanpoucke, Hilde" sort="Vanpoucke, Hilde" uniqKey="Vanpoucke H" first="Hilde" last="Vanpoucke">Hilde Vanpoucke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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